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KMID : 0359319920320010135
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Korean Journal of Veterinary Research
1992 Volume.32 No. 1 p.135 ~ p.142
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In vitro maturation of porcine follicular oocytes
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Abstract
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This experiment was carried out to establish an effective technique of in vitro maturation of porcine follicular oocytes. Porcine ovaries were collected from an abbatoir and delivered to the laboratory in phosphate buffered saline in an hour.
Immatured follicular oocytes were collected from the ovaries and divided into groups by the size of follicles and by the attachment of granulosa cells. The follicular oocytes were cultured in m-KRB solution supplemented with FCS(10%), follicular fluid(10%) or hormones of PMSG(l0IU/㎖), hCG(10IU/㎖ ) and estradiol-17β(1㎍/㎖) for 48 hours at 39℃ under an atmosphere of 5% CO₂ in air.
The results are as follows ;
1. The mean recoveration rate of follicular oocytes was 61.8%.
2. The maturation rate was significantly(p<0.05) higher when the oocytes were collected from large-sized follicles and under good state of granulosa cell attachment.
3. The maturation rate was significantly(p<0.01) promoted when the follicular oocytes were cultured in m-KRB solution supplemented with follicular fluid(74.8%) or hormones and fetal calf serum(70.6%).
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KEYWORD
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